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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1982-12-2
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pubmed:abstractText |
The in vitro preparation of HDL radiolabelled at the cholesteryl ester moiety was determined by incubating different serum fractions with [3H]cholesteryl linoleate or cholesteryl [14C]oleate dissolved in dimethylsulfoxide (DMSO) and subsequent re-isolation of HDL from the incubation mixture by ultracentrifugation. Using the supernatant obtained by precipitation of apolipoprotein B-containing lipoproteins with phosphotungstate/MgCl2 or using the greater than 1.075 kg/l fraction of sera, the bulk of the added radioactivity could be recovered in the re-isolated HDL fraction, as demonstrated by density gradient ultracentrifugation or by gel chromatography. Incubation experiments using whole sera or the greater than 1.063 kg/l fraction of sera resulted in the preferential association of the labelled cholesteryl linoleate with apolipoprotein B-containing lipoproteins.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0009-8981
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
123
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
139-44
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7116635-Apolipoproteins,
pubmed-meshheading:7116635-Apolipoproteins B,
pubmed-meshheading:7116635-Carbon Radioisotopes,
pubmed-meshheading:7116635-Centrifugation, Density Gradient,
pubmed-meshheading:7116635-Chemical Precipitation,
pubmed-meshheading:7116635-Cholesterol Esters,
pubmed-meshheading:7116635-Humans,
pubmed-meshheading:7116635-Isotope Labeling,
pubmed-meshheading:7116635-Lipoproteins, HDL,
pubmed-meshheading:7116635-Male,
pubmed-meshheading:7116635-Oleic Acid,
pubmed-meshheading:7116635-Oleic Acids,
pubmed-meshheading:7116635-Tritium
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pubmed:year |
1982
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pubmed:articleTitle |
Preparation of high-density lipoproteins labelled exclusively at the cholesteryl ester moiety.
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pubmed:publicationType |
Journal Article
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