Linked Life Data

7115725

Source:http://linkedlifedata.com/resource/pubmed/id/7115725

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1982-12-2
pubmed:abstractText
The erythrocruorin of Eisenia fetida possesses a relative molecular mass, determined by sedimentation equilibrium, of (3.82 +/- 0.05) . 10(6). According to the iron and heme contents, 0.218 +/- 0.008% and 2.34 +/- 0.02% by mass, respectively, it contains 144 hemes per molecule. The dimensions of the molecule observed by electron microscopy are 25.0 X 16.5 nm (diameter X height). SDS-polyacrylamide gel electrophoresis indicates that the erythrocruorin consists of six subunits (Mr 14,900, 15,300, 17,200, 19,700, 31,600 and 40,000). Oxygen binding studies showed that E. fetida erythrocruorin has a high oxygen affinity (P50 = 2.8 Torr at pH 7.5), exhibits a slight bohr effect and possesses a high cooperativity with the Hill coefficient h = 3.7-4.8. Treatment of the erythrocruorin either by freezing and thawing or by aging or exposure to alkaline pH converts it irreversibly into a state of lower cooperativity with h = 2.0-2.6. A model of the subunit structure of the erythrocruorin is proposed which takes into account the physiochemical and oxygen-binding properties of the erythrocruorin and the subunits obtained upon its dissociation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
704
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
524-34
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
The erythrocruorin of Eisenia fetida. I. Properties and subunit structure.
pubmed:publicationType
Journal Article