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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1982-10-29
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pubmed:abstractText |
1. The absorption coefficient of human neutrophil plasma-membrane reduced-minus-oxidized cytochrome b-245 was determined [delta epsilon (mM; 559-540 nm) = 21.6 cm-1]. 2. Neutrophil polymorphonuclear leucocytes (neutrophils) were prepared from human, ox, horse and pig blood. In each case plasma-membrane fractions were found to contain low-potential cytochrome b. When membranes from horse neutrophils were incubated anaerobically with either NADH or NADPH the cytochrome b became reduced. Prior stimulation of the cells with phorbol myristate acetate did not increase the rate or extent of cytochrome b reduction in isolated membranes, but did increase both the rate and extent of reduction by NADPH in Triton-treated cells. 3. A cytochrome b was present also in the specific granule fraction of human neutrophils. Its Em (pH 7.0) was found to be -248 mV, very similar to that of the plasma-membrane cytochrome b. 4. The rate of oxidation of reduce cytochrome b-245 by air-saturated buffer, was determined by using stopped-flow techniques. In intact membranes t 1/2 for oxidation was 4.7 ms. This rate is sufficiently rapid to support the view that cytochrome b-245 is the oxidase in the respiratory burst of neutrophils. 5. Plasma-membrane cytochrome b of human neutrophils formed a complex with CO. At room temperature and 1 atm of CO approx. 40% of the cytochrome formed a complex; approx. 60% binding was measured at the increased concentration of dissolved CO achieved at 5 degrees C. The concentration of CO giving 50% binding was 1.18 mM.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7115343-13654378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7115343-13972329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7115343-4289945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7115343-4403603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7115343-454439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7115343-4692017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7115343-6250449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7115343-6261795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7115343-718704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7115343-723935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7115343-7306004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7115343-7306076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7115343-7353656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7115343-7356490
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
204
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
479-85
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7115343-Animals,
pubmed-meshheading:7115343-Carbon Monoxide,
pubmed-meshheading:7115343-Cattle,
pubmed-meshheading:7115343-Cell Membrane,
pubmed-meshheading:7115343-Cytochrome b Group,
pubmed-meshheading:7115343-Cytochromes,
pubmed-meshheading:7115343-Horses,
pubmed-meshheading:7115343-Humans,
pubmed-meshheading:7115343-Kinetics,
pubmed-meshheading:7115343-NADP,
pubmed-meshheading:7115343-Neutrophils,
pubmed-meshheading:7115343-Oxidation-Reduction,
pubmed-meshheading:7115343-Potentiometry,
pubmed-meshheading:7115343-Spectrophotometry,
pubmed-meshheading:7115343-Swine,
pubmed-meshheading:7115343-Tetradecanoylphorbol Acetate
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pubmed:year |
1982
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pubmed:articleTitle |
The enzymic reduction and kinetics of oxidation of cytochrome b-245 of neutrophils.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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