Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-10-12
pubmed:abstractText
Bloodstream forms of Trypanosoma congolense added to the semi-defined medium 109-c (Minimum Essential Medium with Earle's salts, 60 mM HEPES, haemin, and heat-inactivated FBS) and incubated at 28 degrees C transformed into procyclic trypomastigotes. Established cultures of the procyclic forms yielded up to 4.5 X 10(7) parasites/ml. The cultivated populations consisted only of trypomastigotes and were not infective for mice. The amino acids consumed to the greatest extent were glutamine, proline, and threonine. Changes in glucose, pyruvate, and lactate concentrations during growth were determined. The procyclic organisms possessed a well-developed mitochondrion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0044-3255
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
129-35
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Cultivation of procyclic trypomastigotes of Trypanosoma congolense in a semi-defined medium with direct adaptation from bloodstream forms.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't