Linked Life Data

7109583

Source:http://linkedlifedata.com/resource/pubmed/id/7109583

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1982-10-12
pubmed:abstractText
Nuclei from rat ventral prostate were disrupted by sonication and treated with micrococcal nuclease to precipitate nuclear proteins including the androgen receptor. The precipitate was dissolved in 0.6--1.2 M ammonium bicarbonate buffer, pH 7.6, with no loss of receptor when compared to the conventional Tes buffer, pH 7.0 containing 0.6--1.2 M NaCl. Lyophilisation of the solubilised protein did not produce any qualitative or quantitative differences in the recovery of receptor relative to a non-lyophilised control preparation, both of which were analysed for binding properties by Sephadex G-25/G-100 dual-column chromatography. Over longer periods of storage at -80 degrees C, the rate of inactivation of receptor was found to be 6% per week. The stability of the lyophilised receptor was improved by the inclusion of MgCl2 and SH-reducing agents in the ammonium bicarbonate buffer. Recovery improved also with increasing ionic strength of the buffer used to dissolve the lyophilised receptor.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-4731
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
811-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Concentration and preservation of nuclear androgen receptor by lyophilisation.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't