Linked Life Data

7107584

Source:http://linkedlifedata.com/resource/pubmed/id/7107584

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1982-10-12
pubmed:abstractText
Nonmuscle, smooth muscle, and striated muscle tissue extracts contain several proteins, in addition to calmodulin, which bind fluphenazine affinity columns in a calcium-dependent manner. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis shows four proteins in red blood cell lysates with Mr = 22,000, 12,000, 9,000, and 8,000. Rat brain tissue contains an 11,000-dalton peptide, while chicken gizzard and rabbit longissimus dorsi muscles have a similar set of peptides (67,000, 35,000, 33,000, and 11,000 daltons). These proteins all interact independently with fluphenazine and, except for the 22,000-dalton protein from red blood cells, do not bind to calmodulin-Sepharose affinity columns. Binding requires the presence of micromolar calcium, and dissociation occurs only by chelation of calcium with EDTA or ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid. The muscle tissue proteins are present in ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid eluates from N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide- and phenothiazine-coupled Sepharose resins but are not bound to a Sepharose column without a coupled drug. These results suggest that the use of phenothiazines to indicate calmodulin action should be judiciously interpreted. These proteins may be functionally analogous to calmodulin in that they form a drug-binding site in a calcium-dependent manner.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
257
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9663-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Calcium-dependent protein binding to phenothiazine columns.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.