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pubmed:abstractText |
Methanobacterium bryantii, grown autotrophically on H2-CO2, transported nickel against a concentration gradient by a high-affinity system (Km = 3.1 microM). The system had a pH optimum of 4.9 and a temperature optimum of 49 degrees C with an energy of activation of 7.8 kcal/mol (ca. 32.6 kJ/mol). A headspace of H2-CO2 (4:1, vol/vol) was required for maximum rate of transport. The system was highly specific for nickel and was unaffected by high levels of all monovalent and divalent ions tested (including Mg2+) with the sole exception of Co2+. Kinetic experiments indicated that accumulated nickel became increasingly incorporated into cofactor F430 and protein. Nickel transport was inhibited by nigericin, monensin, and gramicidin but not by carbonyl cyanide-p-trifluoromethoxyphenyl hydrazone, carbonyl cyanide-m-chlorophenyl hydrazone, N,N'-dicyclohexylcarbodiimide, valinomycin plus potassium, or acetylene. The ineffectiveness of carbonyl cyanide-p-trifluoromethoxyphenyl hydrazone, carbonyl cyanide-m-chlorophenyl hydrazone, and N,N'-dicyclohexylcarbodiimide may be related to difficulties in the penetration of these compounds through the outer cell barriers. Nickel uptake was greatly stimulated by an artificially imposed pH gradient (inside alkaline). The data suggest that nickel transport is not dependent on the membrane potential or on intracellular ATP, but is coupled to proton movement.
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