rdf:type |
|
lifeskim:mentions |
umls-concept:C0008328,
umls-concept:C0030685,
umls-concept:C0032105,
umls-concept:C0034580,
umls-concept:C0086418,
umls-concept:C0391871,
umls-concept:C0449432,
umls-concept:C0542502,
umls-concept:C0680255,
umls-concept:C1179435,
umls-concept:C1283071,
umls-concept:C1521991,
umls-concept:C1524073,
umls-concept:C1548799,
umls-concept:C1705248,
umls-concept:C1963578
|
pubmed:issue |
3
|
pubmed:dateCreated |
1982-10-21
|
pubmed:abstractText |
The preparation of a stable fully immunoreactive 125I-labeled CCK39 using a modified Iodo-gen method with high specific radioactivity; the production of an avid and specific cholecystokinin (CCK) antiserum, and a sensitive, precise and specific radioimmunoassay method allowing measurements of fasting plasma CCK in the low picomole per liter range together with the significant rises in plasma CCK following a test meal and duodenal infusion of fat are described. Apparent immunoreactive fasting plasma CCK was eluted from a Sephadex G-50 Fine column in one peak probably representing plasma CCK bound to plasma proteins and nonspecific plasma effects. Apparent immunoreactive postprandial plasma CCK was eluted from a Sephadex G-50 Fine column in four peaks. The first peak probably represents plasma CCK bound to plasma proteins and nonspecific plasma effects; the second peak probably represents component I with a molecular weight between some 5,000 and 30,000; the third peak probably represents component II or CCK33, and the fourth peak probably represents component IV or CCK8.
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pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,3,4,6-tetrachloro-3 alpha,6...,
http://linkedlifedata.com/resource/pubmed/chemical/Cholecystokinin,
http://linkedlifedata.com/resource/pubmed/chemical/Corn Oil,
http://linkedlifedata.com/resource/pubmed/chemical/Dietary Fats,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Oils,
http://linkedlifedata.com/resource/pubmed/chemical/Urea
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pubmed:status |
MEDLINE
|
pubmed:issn |
0012-2823
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
23
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
156-68
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:7106417-Adult,
pubmed-meshheading:7106417-Animals,
pubmed-meshheading:7106417-Cholecystokinin,
pubmed-meshheading:7106417-Corn Oil,
pubmed-meshheading:7106417-Dietary Fats,
pubmed-meshheading:7106417-Duodenum,
pubmed-meshheading:7106417-Humans,
pubmed-meshheading:7106417-Imidazoles,
pubmed-meshheading:7106417-Immune Sera,
pubmed-meshheading:7106417-Indicators and Reagents,
pubmed-meshheading:7106417-Iodine Radioisotopes,
pubmed-meshheading:7106417-Isotope Labeling,
pubmed-meshheading:7106417-Male,
pubmed-meshheading:7106417-Oils,
pubmed-meshheading:7106417-Rabbits,
pubmed-meshheading:7106417-Radioimmunoassay,
pubmed-meshheading:7106417-Swine,
pubmed-meshheading:7106417-Urea
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pubmed:year |
1982
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pubmed:articleTitle |
Iodination with Iodo-gen and radioimmunoassay of cholecystokinin (CCK) in acidified plasma, CCK release, and molecular CCK components in man.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|