Linked Life Data

7099139

Source:http://linkedlifedata.com/resource/pubmed/id/7099139

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-9-24
pubmed:abstractText
Neuroblastoma cells clone N-2a, differentiated by serum deprivation, were found to take up tritiated serotonin ([3H]5-HT) from the external medium by means of a saturable mechanism which follows Michaelis-Menten kinetics. The apparent Km of uptake was 1.27 microM and the Vmax 720 fmoles/min/10(6) cells. The uptake was temperature-dependent and partially sodium-dependent, and was inhibited by ouabain and by selected metabolic inhibitors (sodium azide, 2,4-dinitrophenol, and iodoacetamide). Fluoxetine and desmethylimipramine (DMI) were equally effective inhibitors of [3H]5-HT uptake (IC50 = 13.7 microM and 13.6 microM). The uptake was structurally specific, since unlabeled 5-HT was a better inhibitor of [3H]5-HT uptake than norepinephrine (NE) (IC 50 = 0.6 microM and 9.4 microM). The neurotoxins 6-hydroxydopamine and 5,6-dihydroxytryptamine were cytotoxic to differentiated N-2a cells, causing time- and concentration-dependent inhibition of [3H]thymidine incorporation into DNA, 5,7-Dihydroxytryptamine had little cytotoxic effect. Non-differentiated N-2a cells, supplemented with 5% fetal calf serum, were also found to take up [3H]5-HT by a concentration-, temperature-, and energy-dependent process. The apparent Km of uptake was 0.96 microM and the Vmax was 619 fmoles/min/10(6) cells. However, in nondifferentiated cells [3H]5-HT uptake was not sodium-dependent, not inhibited by ouabain, less effectively inhibited by fluoxetine and DMI (IC50 = 148 microM and 107 microM), and not selectively inhibited by unlabeled 5-HT as compared with NE (IC50 = 7.9 microM and 6.0 microM).
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0026-895X
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
362-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Characterization of serotonin uptake in cultured neuroblastoma cells. Difference between differentiated and nondifferentiated cells.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't