Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1982-9-10
pubmed:abstractText
Herpesvirus sylvilagus was propagated in juvenile cotton tail rabbit kidney cells and purified from the cytoplasmic fraction of the infected cells. The purification procedure included zonal centrifugation through a 5 to 30% dextran t-10 gradient, followed by equilibrium centrifugation in a 5 to 50% potassium tartrate gradient. H. sylvilagus formed one band after centrifugation through the tartrate gradient at a density of 1.22 g/cm3. Contamination of the purified virus preparation by cellular proteins was less than 0.2% as determined by the removal of radioactivity from an artificially mixed sample containing [35S]methionine-labeled control cells and nonlabeled infected cells. H. sylvilagus nucleocapsids were isolated from infected cell nuclei and purified by sedimentation through a 36% sucrose cushion, followed by equilibrium centrifugation in 5 to 50% tartrate gradient. Forty-four polypeptides ranging in molecular weight from 18,000 to 230,00 were resolved when [35S]methionine-labeled enveloped H. sylvilagus was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Seventeen polypeptides found within the enveloped virus were also identified with the nucleocapsid. Six additional nucleocapsid polypeptides han no counterparts within the enveloped virus. The major polypeptide within both the virus and the nucleocapsid had a molecular weight of 150,000.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-1167720, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-13675766, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-16557976, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-170424, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-176450, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-176452, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-176465, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-186638, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-211256, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-220786, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-227161, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-4110104, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-4333738, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-4337890, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-4344252, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-4351372, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-4352378, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-4418750, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-5137313, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-5462264, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-5806584, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-6154808, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-6251617, http://linkedlifedata.com/resource/pubmed/commentcorrection/7097852-6252328
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1063-72
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Herpesvirus sylvilagus I. Polypeptides of virions and nucleocapsids.
pubmed:publicationType
Journal Article