Linked Life Data

7093726

Source:http://linkedlifedata.com/resource/pubmed/id/7093726

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-9-10
pubmed:abstractText
Light microscope radioautographic procedures were employed to investigate the binding patterns of lectins derived from Lotus tetragonolobus (Lotus A) and Ulex europeus (UEA I) to the surfaces of rat cerebellar cells maintained in dispersed cell culture. Lotus A bound extensively to the surfaces of neuronal cell somata and neuronal processes at all time points investigated; the lectin also bound to nonneuronal cell surfaces, but to an extent much less than that for neurons. Lectin binding was completely inhibited by the presence of 0.1 M alpha-L-fucose in the reaction medium. Conversely, UEA I did not appear to bind to any significant extent to the surfaces of cerebellar cells in vitro at any stage of maintenance. The incorporation of [3H]fucose into fucosyl-conjugates by cerebellar neurons in culture was investigated using light microscope radioautography and polyacrylamide gel electrophoretic methods. Radioautographs indicated that the fucose came to be distributed throughout neuronal cell somata and processes and that, with increasing time, the grain density appeared to be highest over areas of neuropil and fascicles of cellular processes. The electrophoresis data demonstrated that, among others, the label was incorporated into prominent classes of polypeptides having nominal molecular weights of 400,000, 125,000, 91,000 and 46,000 daltons.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:volume
256
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13-21
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Distribution of fucosyl-conjugates in rat cerebellar cells in vitro: binding patterns of Lotus tetragonolobus and Ulex europeus lectins.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.