Linked Life Data

7093215

Source:http://linkedlifedata.com/resource/pubmed/id/7093215

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1982-9-24
pubmed:abstractText
It is shown, by means of analytical ultracentrifugation, that skeletal myosin S-1 exists in the form of a monomer-dimer mixture, in rapid reversible equilibrium, sensitive to the hydrostatic pressure, the temperature, and the composition of the buffer (at least, pH, ionic strength, presence or absence of a Mg-(phosphate compound), and presence or absence of Mg2+). The dimer is predominant at high pH, at low ionic strength, in the presence of a Mg-(phosphate compound), at high pressure, and at low temperature. The monomer is predominant in the reverse conditions. At atmospheric pressure and at room temperature, in a buffer having a composition close to that of the physiological medium, but containing no Mg-(phosphate compound), the monomer is largely predominant (more than 90% at 1 mg/mL S-1). At atmospheric pressure and at room temperature, in a buffer containing a Mg-(phosphate compound) and having a composition close to that of the physiological medium, S-1 exists in the form of a monomer-dimer mixture, with a noticeable proportion of dimer (more than 25% at 1 mg/mL S-1 in the presence of 2 mM MgADP and 3 mM Mg2+). In such buffers, the monomer:dimer ratio is extremely sensitive to both the pH and the ionic strength. The sedimentation coefficients of the monomer and the dimer are respectively 5.05 +/- 0.05 S and 6.05 +/- 0.05 S. The two protomers making up the dimer are stuck together in an end-to-end arrangement. Both the monomer and the dimer are highly hydrated (about 0.9 g of water/g of protein for the monomer and probably more for the dimer).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2679-86
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Dimerization of the myosin heads in solution.
pubmed:publicationType
Journal Article