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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1982-8-7
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pubmed:abstractText |
This study was undertaken to determine if rabbit neutrophils contain sufficient ether-linked precursor for the synthesis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor) by a deacylation-reacylation pathway. The phospholipids from rabbit peritoneal polymorphonuclear neutrophils were purified and quantitated, and the choline-containing and ethanolamine-containing phosphoglycerides were analyzed for ether lipid content. Choline-containing phosphoglycerides (37%), ethanolamine-containing phosphoglycerides (30%), and sphingomyelin (28%) were the predominant phospholipid classes, with smaller amounts of phosphatidylserine (5%) and phosphatidylinositol (less than 1%). The choline-linked fraction contained high amounts of 1-O-alkyl-2-acyl-(46%) and 1,2-diacyl-sn-glycero-3-phosphocholine (54%), with a trace of the 1-O-alk-1'-enyl-2-acyl species. The ethanolamine-linked fraction contained high amounts of 1-O-alk-1'-enyl-2-acyl-(63%) and 1,2-diacyl-sn-glycero-3-phosphoethanolamine (34%), and a low quantity of the 1-O-alkyl-2-acyl species (3%). The predominant 1-O-alkyl ether chains found in the sn-1 position of the choline-linked fraction were 16:0 (35%), 18:0 (14%), 18:1 (26%), 20:0 (16%), and 22:0 (9%). The major 1-O-alk-1'-enyl ether chains found in the sn-1 position of the ethanolamine-linked fraction were 14:0 (13%), 16:0 (44%), 18:0 (27%), 18:1 (12%) and 18:2 (3%). The major acyl groups in the sn-1 position of 1,2-diacyl-sn-glycero-3-phosphocholine and 1,2-diacyl-sn-glycero-3-phosphoethanolamine were 16:0, 18:0 and 18:1. The most abundant acyl group in the sn-2 position of all classes of choline- and ethanolamine-linked phosphoglycerides was 18:2. Although this work does not define the biosynthetic pathway for platelet activating factor, it does show that there is ample precursor present to support its synthesis by a deacylation-reacylation pathway.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Lysophosphatidylcholines,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholines,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylethanolamines,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Activating Factor
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0024-4201
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
72-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7087685-Animals,
pubmed-meshheading:7087685-Fatty Acids,
pubmed-meshheading:7087685-Lysophosphatidylcholines,
pubmed-meshheading:7087685-Mass Spectrometry,
pubmed-meshheading:7087685-Neutrophils,
pubmed-meshheading:7087685-Phosphatidylcholines,
pubmed-meshheading:7087685-Phosphatidylethanolamines,
pubmed-meshheading:7087685-Phospholipids,
pubmed-meshheading:7087685-Platelet Activating Factor,
pubmed-meshheading:7087685-Rabbits
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pubmed:year |
1982
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pubmed:articleTitle |
Ether lipid content and fatty acid distribution in rabbit polymorphonuclear neutrophil phospholipids.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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