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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1982-8-14
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pubmed:abstractText |
Mouse L cells transformed with recombinant plasmids carrying hepatitis B virus (HBV) DNA fragments were used to study the transcription of the viral surface antigen gene (gene S). An HBV-specific, polyadenylated, 2.3-kilobase RNA was mapped on the HBV genome. This RNA hybridized with approximately 75% of the genome and excluded the region of the HBV core antigen gene (gene C). The 2.3-kilobase RNA species was present only in cell lines that produced hepatitis B surface antigen. An HBV-specific 2.3-kilobase RNA was also detected in human hepatoma cell line PLC/PRF/5 which produced hepatitis B surface antigen. A study of gene S expression in the transformed mouse L cells allowed us to localize the regions of initiation and termination of gene S transcription. Our results strongly suggest that the 2.3-kilobase RNA molecule is the mRNA of the major polypeptide of the envelope, which carries the viral surface antigen determinants.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-226978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-274719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-291033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-399327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-399329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-551290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-6159641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-6250073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-6250074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-6250075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-6254085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-6259538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-6264484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-6264599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-63998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-6933503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7086959-73185
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
100-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7086959-Animals,
pubmed-meshheading:7086959-Cloning, Molecular,
pubmed-meshheading:7086959-DNA, Viral,
pubmed-meshheading:7086959-Genes, Viral,
pubmed-meshheading:7086959-Hepatitis B Surface Antigens,
pubmed-meshheading:7086959-Hepatitis B virus,
pubmed-meshheading:7086959-L Cells (Cell Line),
pubmed-meshheading:7086959-Mice,
pubmed-meshheading:7086959-Operon,
pubmed-meshheading:7086959-Plasmids,
pubmed-meshheading:7086959-RNA, Messenger,
pubmed-meshheading:7086959-RNA, Viral,
pubmed-meshheading:7086959-Transcription, Genetic,
pubmed-meshheading:7086959-Transformation, Genetic
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pubmed:year |
1982
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pubmed:articleTitle |
Transcription of the hepatitis B surface antigen gene in mouse cells transformed with cloned viral DNA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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