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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1982-8-26
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pubmed:abstractText |
A simple and sensitive method is described for the detection of cell surface proteins. This method consists of reacting concanavalin A (Con A) with 35S-labeled methionine, detergent solubilized whole cell extracts and immunoprecipitating the Con A-protein complexes with anti-Con A antibody bound to Staphylococcus aureus Cowan I strain (SACI). These reagents are commercially available, stable and easy to standardize. Mouse lymphocytes cell surface proteins purified by this procedure show great similarities to those purified with goat anti-cell surface antibodies.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0022-1759
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
213-20
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:7086153-Animals,
pubmed-meshheading:7086153-Electrophoresis, Agar Gel,
pubmed-meshheading:7086153-Female,
pubmed-meshheading:7086153-Glycoproteins,
pubmed-meshheading:7086153-Lymphocytes,
pubmed-meshheading:7086153-Male,
pubmed-meshheading:7086153-Membrane Proteins,
pubmed-meshheading:7086153-Mice,
pubmed-meshheading:7086153-Mice, Inbred C57BL,
pubmed-meshheading:7086153-Receptors, Concanavalin A
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pubmed:year |
1982
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pubmed:articleTitle |
A simple method for electrophoretic analysis of cell surface glycoproteins based on concanavalin A binding.
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pubmed:publicationType |
Journal Article
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