Linked Life Data

7086126

Source:http://linkedlifedata.com/resource/pubmed/id/7086126

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-8-7
pubmed:abstractText
Two isoallergenic forms of short ragweed (Ambrosia elatior) allergen Ra5 were isolated in ultrapure form by a combination of molecular sieving (through Amicon hollow-fiber cartridges), cation-exchange chromatography, and gel filtration. The two forms, Ra5A and Ra5B, exhibited charge differences by CM-Sephadex chromatography and agarose electrophoresis at pH 8.5. The A form as identical to conventional Ra5 by its physical and chemical properties and was more cationic than the previously undescribed B form. Ra5A and Ra5B had identical m.w. of 5000 by SDS-PAGE. Their amino-terminal sequences of 30 residues showed no differences from each other or from the previously published sequence of the major form of Ra5. The two forms were antigenically indistinguishable when using hyperimmunized animal antisera to Ra5 and crude ragweed extract. Although the IgG and IgE ab of most Ra5-responder patients could not discriminate between the two Ra5 forms, significant small differences in binding to Ra5A and Ra5B were consistently observed in 10 to 15% of the patients studied. Further analysis of the Ra5A preparation suggested a purity of at least 99.5% and probably greater than or equal to 99.9%.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:volume
129
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
120-5
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Two isoallergens of short ragweed component Ra5.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.