Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-8-26
pubmed:abstractText
In a previous study, a seronegative child to whom attenuated A/Alaska/77-ts-1A2 virus was administered (37 degrees C shutoff temperature for plaque formation) shed virus with an altered temperature-sensitive (ts) phenotype (40 degrees C shutoff temperature) (Murphy et al., Ann. N.Y. Acad. Sci. 354:172-182, 1980; Tolpin et al., Virology 112:505-517, 1981). This ts+ virus (FV1319) was evaluated for its level of replication in hamsters and for its virulence for humans. In hamsters, FV1319 ts+ virus replicated to the same level in the nasal turbinates as that of which the A/Alaska/77 wild-type virus replicated, but its replication in the lungs was reduced 40-fold. In contrast, the A/Alaska/77-ts-1A2 reassortant achieved a titer in hamster nasal turbinates that was significantly lower (P less than 0.005) than those achieved by the wild-type and the FV1319 viruses; the A/Alaska/77-ts-1A2 reassortant was not recoverable from the lungs. In seronegative adult volunteers, the pattern of replication of the FV1319 virus was similar to that of the A/Alaska/77 wild-type virus. The illness induced by the FV1319 ts+ virus was also similar to that caused by the wild-type virus. In contrast, the A/Alaska/77-ts-1A2 reassortant was satisfactorily attenuated in adult volunteers. These results suggest that attenuation of the A/Alaska/77-ts-1A2 reassortant virus in humans is a function of the ts phenotype: loss of this phenotype restored virulence. The ability of the A/Alaska/77-ts-1A2 reassortant to lose its ts phenotype and regain virulence during growth in a permissive host limits the usefulness of the ts-1A2 reassortants as vaccine viruses for humans.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-1003014, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-1083310, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-1272630, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-167681, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-399362, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-422239, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-5137591, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-597038, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-669818, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-669819, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-6893373, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-6939404, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-695326, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-695327, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-6997969, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-7011972, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-7216417, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-7257183, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-7399695, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-7425857, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-7425858, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-810557, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-894078, http://linkedlifedata.com/resource/pubmed/commentcorrection/7085075-929981
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
645-50
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Evaluation of a phenotypic revertant of the A/Alaska/77-ts-1A2 reassortant virus in hamsters and in seronegative adult volunteers: further evidence that the temperature-sensitive phenotype is responsible for attenuation of ts-1A2 reassortant viruses.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.