| pubmed-article:7084300 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7084300 | lifeskim:mentions | umls-concept:C0007450 | lld:lifeskim |
| pubmed-article:7084300 | lifeskim:mentions | umls-concept:C0080103 | lld:lifeskim |
| pubmed-article:7084300 | lifeskim:mentions | umls-concept:C0006104 | lld:lifeskim |
| pubmed-article:7084300 | lifeskim:mentions | umls-concept:C0205147 | lld:lifeskim |
| pubmed-article:7084300 | lifeskim:mentions | umls-concept:C0034798 | lld:lifeskim |
| pubmed-article:7084300 | lifeskim:mentions | umls-concept:C0037956 | lld:lifeskim |
| pubmed-article:7084300 | lifeskim:mentions | umls-concept:C0441472 | lld:lifeskim |
| pubmed-article:7084300 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:7084300 | lifeskim:mentions | umls-concept:C0475264 | lld:lifeskim |
| pubmed-article:7084300 | lifeskim:mentions | umls-concept:C0146951 | lld:lifeskim |
| pubmed-article:7084300 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:7084300 | pubmed:dateCreated | 1982-8-7 | lld:pubmed |
| pubmed-article:7084300 | pubmed:abstractText | [3H]Spiroperidol binding was used to assay dopaminergic receptors in synaptosomal membranes isolated from basal ganglia of cat brain. In addition to the usual filtration, a special centrifugation technique was developed which has some advantages for ligands with high non-specific binding. The specific binding to control membranes showed a high affinity binding site with KD 0.99 nM and Bmax 445 fmol/mg protein, both determined by Scatchard analysis. Addition of 10(-5) -10(-3)% Triton X-100 increased the Bmax of the high affinity binding site without a change in KD. With higher concentrations of detergent (10(-2) -10 (-1)% there was inhibition of binding. Both the activation and the inhibition of binding were reversed by washing of the detergent; but the reversal of the activation was accompanied by a decrease in affinity. The binding to synaptosomal membranes treated with 10(-1) - 10(-2)% Triton X-100 and then washed led to a loss of receptors probably present in the presynaptic membrane. These and other results suggest that dopaminergic receptors are localized both pre- and postsynaptically in relation to the synaptic region. | lld:pubmed |
| pubmed-article:7084300 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7084300 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7084300 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7084300 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7084300 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7084300 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7084300 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7084300 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7084300 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7084300 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:7084300 | pubmed:issn | 0014-2999 | lld:pubmed |
| pubmed-article:7084300 | pubmed:author | pubmed-author:De RobertisEE | lld:pubmed |
| pubmed-article:7084300 | pubmed:author | pubmed-author:Jimenez... | lld:pubmed |
| pubmed-article:7084300 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7084300 | pubmed:day | 8 | lld:pubmed |
| pubmed-article:7084300 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:7084300 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7084300 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7084300 | pubmed:pagination | 1-9 | lld:pubmed |
| pubmed-article:7084300 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:7084300 | pubmed:meshHeading | pubmed-meshheading:7084300-... | lld:pubmed |
| pubmed-article:7084300 | pubmed:meshHeading | pubmed-meshheading:7084300-... | lld:pubmed |
| pubmed-article:7084300 | pubmed:meshHeading | pubmed-meshheading:7084300-... | lld:pubmed |
| pubmed-article:7084300 | pubmed:meshHeading | pubmed-meshheading:7084300-... | lld:pubmed |
| pubmed-article:7084300 | pubmed:meshHeading | pubmed-meshheading:7084300-... | lld:pubmed |
| pubmed-article:7084300 | pubmed:meshHeading | pubmed-meshheading:7084300-... | lld:pubmed |
| pubmed-article:7084300 | pubmed:meshHeading | pubmed-meshheading:7084300-... | lld:pubmed |
| pubmed-article:7084300 | pubmed:meshHeading | pubmed-meshheading:7084300-... | lld:pubmed |
| pubmed-article:7084300 | pubmed:meshHeading | pubmed-meshheading:7084300-... | lld:pubmed |
| pubmed-article:7084300 | pubmed:meshHeading | pubmed-meshheading:7084300-... | lld:pubmed |
| pubmed-article:7084300 | pubmed:meshHeading | pubmed-meshheading:7084300-... | lld:pubmed |
| pubmed-article:7084300 | pubmed:meshHeading | pubmed-meshheading:7084300-... | lld:pubmed |
| pubmed-article:7084300 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:7084300 | pubmed:articleTitle | Dopaminergic receptors in cat brain. Action of triton X-100 on [3H]spiroperidol binding and localization in relation to the synaptic region. | lld:pubmed |
| pubmed-article:7084300 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7084300 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:7084300 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7084300 | lld:pubmed |
