Linked Life Data

7084300

Source:http://linkedlifedata.com/resource/pubmed/id/7084300

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1982-8-7
pubmed:abstractText
[3H]Spiroperidol binding was used to assay dopaminergic receptors in synaptosomal membranes isolated from basal ganglia of cat brain. In addition to the usual filtration, a special centrifugation technique was developed which has some advantages for ligands with high non-specific binding. The specific binding to control membranes showed a high affinity binding site with KD 0.99 nM and Bmax 445 fmol/mg protein, both determined by Scatchard analysis. Addition of 10(-5) -10(-3)% Triton X-100 increased the Bmax of the high affinity binding site without a change in KD. With higher concentrations of detergent (10(-2) -10 (-1)% there was inhibition of binding. Both the activation and the inhibition of binding were reversed by washing of the detergent; but the reversal of the activation was accompanied by a decrease in affinity. The binding to synaptosomal membranes treated with 10(-1) - 10(-2)% Triton X-100 and then washed led to a loss of receptors probably present in the presynaptic membrane. These and other results suggest that dopaminergic receptors are localized both pre- and postsynaptically in relation to the synaptic region.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0014-2999
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Dopaminergic receptors in cat brain. Action of triton X-100 on [3H]spiroperidol binding and localization in relation to the synaptic region.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't