Linked Life Data

7083304

Source:http://linkedlifedata.com/resource/pubmed/id/7083304

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-8-26
pubmed:abstractText
Spermathecal accessory glands from pupae of Tenebrio molitor were cultured in vitro in Landureau S-20 medium with or without ecdysterone at a concentration of 5 micrograms/ml medium. Morphological changes were examined by electron microscopy. Tissue taken from pupae that have not been exposed to a peak of ecdysterone in vivo is only able to differentiate in medium with hormone, and then only partially, while tissue taken from pupae that have experienced an endogenous peak of ecdysterone is able to develop maximally irrespective of the presence or absence of hormones. The specific ultrastructural changes that occur in vitro correspond to those occurring in the gland in situ during the normal course of differentiation, and are: the formation of the pseudocilium, cell retraction and formation of an end apparatus, and cuticulogenesis in the ductule and main lumen of the gland. Pseudocilium formation does not appear to be ecdysterone-dependent, while cuticulogenesis requires ecdysterone for initiation of the process. Deposition of cuticle is an expression of an earlier commitment of the cells to this process, and is initiated by elevated levels of ecdysterone. Ecdysterone is not required for completion of cuticulogenesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0302-766X
pubmed:author
pubmed:issnType
Print
pubmed:volume
222
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
269-81
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Cytodifferentiation of the accessory glands of Tenebrio molitor. IX. differentiation of the spermathecal accessory gland in vitro.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.