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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1982-7-8
|
| pubmed:abstractText |
The acid deoxyribonuclease was isolated from Bombyx mori eggs and its physico-chemical properties were investigated. The enzyme purified 160-fold did not contain admixtures of phosphomono- and phosphodiesterases or ribonuclease. The molecular weight of the enzyme is 40 000 +/- 1000, isoelectric point lies at 6.5. The maximum activity is revealed at pH 5.2, 50 degrees. The DNAase is insignificantly activated by Mg2+ and is inhibited by Cu2+ and Zn2+. The enzyme preferentially hydrolyzes native DNA and is an endonuclease splitting DNA down to 5'-oligonucleotides.
|
| pubmed:language |
rus
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0320-9725
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
47
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
489-96
|
| pubmed:dateRevised |
2007-7-23
|
| pubmed:meshHeading |
pubmed-meshheading:7074175-Animals,
pubmed-meshheading:7074175-Bombyx,
pubmed-meshheading:7074175-Cations, Divalent,
pubmed-meshheading:7074175-Deoxyribonucleases,
pubmed-meshheading:7074175-Female,
pubmed-meshheading:7074175-Hydrogen-Ion Concentration,
pubmed-meshheading:7074175-Kinetics,
pubmed-meshheading:7074175-Molecular Weight,
pubmed-meshheading:7074175-Ovum
|
| pubmed:year |
1982
|
| pubmed:articleTitle |
[Isolation, purification and properties of acid deoxyribonuclease from silkworm Bombyx mori eggs].
|
| pubmed:publicationType |
Journal Article,
English Abstract
|