Linked Life Data

7074011

Source:http://linkedlifedata.com/resource/pubmed/id/7074011

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-7-19
pubmed:abstractText
The size and shape of bovine cardiac troponin C (TN-C) in solution have been examined by gel filtration, ultracentrifugation, and viscosity in the presence and absence of Ca2+. Cardiac TN-C (-Ca2+) has an intrinsic sedimentation coefficient, s020,w, of 1.87 S and a Stokes radius, RS, of 26.3 A as determined by gel chromatography on Sephacryl S-300. In 2 mM Ca2+, S020,w is increased to 2.04 S and RS is decreased to 24.3 A, indicating a conformational change to a more compact structure. Furthermore, the intrinsic viscosity of TN-C in the absence of Ca2+ (6.4 mL/g) is reduced to 5.4 mL/g when Ca2+ is added. Sedimentation equilibrium studies indicate that the effects of Ca2+ are not due to changes in the molecular weight of the protein. The hydrodynamic data suggest that TN-C is a moderately asymmetric protein with an axial ratio of 4--6.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
229-33
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Hydrodynamic properties of bovine cardiac troponin C.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't