Linked Life Data

7062743

Source:http://linkedlifedata.com/resource/pubmed/id/7062743

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-5-27
pubmed:abstractText
Intact human lymphocytes were incubated with tritium-labeled androstenedione; after extraction and appropriate chromatographic separation, followed by crystallization to constant tritium to carbon-14 ratio to establish radiochemical homogeneity, the following metabolites were identified: testosterone, 5 alpha-androstane-3,17-dione, 5 alpha-dihydrotestosterone, androsterone and isoandrosterone. Neither estrogen nor 5 beta-reduced metabolites were found as products. The major androstenedione metabolizing activities in human lymphocytes were 5 alpha-reductase and 17 beta-hydroxysteroid oxidoreductase; in addition, 3 alpha-and 3 beta-hydroxysteroid oxidoreductase activities also were present in these cells. The rates of metabolite formation were linear with incubation time up to 4 h, and with cell number up to 2 x 10(7) lymphocytes per ml for the 5 alpha-reduced products and up to 1.6 x 10(7) lymphocytes per ml for testosterone. These findings are suggestive that, in the human, lymphocytes are a potential site of peripheral metabolism of androstenedione to potent adnrogens.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-4731
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Androstenedione metabolism by human lymphocytes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.