Linked Life Data

7062062

Source:http://linkedlifedata.com/resource/pubmed/id/7062062

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1982-5-27
pubmed:abstractText
The effect of thiol reagents on the specific binding of the atypical neuroleptic, sulpiride, to rat striatal membranes are examined. Pretreatment of membranes with N-ethylmaleimide (NEM), but not with iodoacetamide or dithiothreitol (DTT), diminishes [3H]sulpiride binding. The effect is dependent on time, temperature, and the concentration of NEM. The reaction proceeds with pseudo-first-order rate kinetics, indicating the involvement of a single essential SH group in the binding of [3H]sulpiride to striatal membranes sites. Both sodium and sulpiride, but not nucleotides, protect the [3H]sulpiride sites from NEM inactivation in a dose-dependent fashion. As sodium is essential for [3H]sulpiride binding it is possible that in the presence of sodium and sulpiride a conformational change takes place that makes the essential SH group less accessible to alkylation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1459-65
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Influence of sodium and sulphydryl groups on [3H]sulpiride binding sites in rat striatal membranes.
pubmed:publicationType
Journal Article