Linked Life Data

7061510

Source:http://linkedlifedata.com/resource/pubmed/id/7061510

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1982-5-27
pubmed:abstractText
The structures of O-glycosidically linked oligosaccharides from plasma membranes of AH 66 ascites hepatoma cells have been determined. Glycopeptides were prepared from a Triton X-100 extract of the cells by pronase digestion. From the digest, the glycopeptide containing O-glycosidically linked oligosaccharides was isolated by gel filtration and electrophoresis on a cellulose acetate block. The oligosaccharide chains were isolated as reduced oligosaccharides by treating the glycopeptide with alkaline borohydride. Neutral and acidic oligosaccharides were separated on a column of Dowex 1, and then each of them was further fractionated by gel filtration on Sephadex G-25 (superfine), high voltage paper electrophoresis, and paper chromatography. One reduced monosaccharide, three neutral, and five acidic reduced oligosaccharides were isolated. On the basis of carbohydrate compositions, mass spectra of permethylated oligosaccharides, methylation analysis, and exoglycosidase digestion, their structures were deduced to be as follows: (formula, see text).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
257
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3782-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Structure of O-glycosidically linked sugar units from plasma membranes of an ascites hepatoma, AH 66.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't