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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1982-5-21
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pubmed:abstractText |
O2 tensions (Po2) were measured with microelectrodes in multicellular spheroids from EMT6/Ro and V-79-171-B cells. The measurements were performed in spheroids kept in flowing growth medium that was equilibrated with 5% CO2 and air at a temperature of 37 degrees C and contained 5.5 mM glucose. The recorded Po2 profiles are characterized by a diffusion-depleted zone surrounding the spheroids and by a steep drop in Po2 within the spheroids over mean distance of 220 and 188 micrometer from the surface of EMT6/Ro and V-79-171B spheroids over mean distance of 220 and 188 micrometer from the surface of EMT6/Ro and V-79-171B spheroids respectively. Smaller spheroid exhibit parabolic Po2 profiles, larger ones show a central plateau. The region of the steep decrease in Po2 corresponds to the thickness of the viable rim: the plateau region is created by the absence of O2 consumption in the central necrotic area. Po2 in the centre of EMT6/Ro spheroids decreased from 66 mmHg at a diameter of 400 micrometer to 13 mmHg at a diameter of 1000 micrometer. Under the present conditions during growth and in the experiments, values below 5 mmHg were recorded only in spheroids 1200 micrometer. Comparably low Po2 was recorded in V-79 spheroids with diameters of 650 micrometer +. In spheroids of this cell type with a diameter of 400 micrometer, Po2 was 42 mmHg. The findings provide evidence that necrosis may arise at average Po2 of 57 and 42 mmHg in EMT6/Ro and V-79-171B spheroids, respectively, grown under the conditions described.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-1035033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-128440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-13584703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-13622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-13895456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-16744238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-277219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-327212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-449723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-4540603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-4709724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-4743388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-482606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-482607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-5067983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-5101993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-544580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-6054008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-670026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-7249257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-7319864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-734056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-7471046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7059474-9393
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0007-0920
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
256-64
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1982
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pubmed:articleTitle |
Oxygen tensions in multicell spheroids of two cell lines.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Historical Article
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