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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1982-2-25
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pubmed:abstractText |
Villin, a 95,000-dalton protein, is a major component of microvillus cores isolated from intestinal brush borders. In this study, we compared the Ca2+-dependent action of this protein on actin filaments with that of cytochalasin B, a fungal metabolite that binds to the "barbed" end of actin filaments and nuclei. We found that substoichiometric levels of villin inhibit actin filament elongation and self-association in a cytochalasin-like manner. In addition, the protein releases membrane-bound F-actin in the absence of high shear force, probably by severing the filaments. The filament fragments formed in the presence of villin, as well as a nucleating complex consisting of villin and actin, bind stoichiometric amounts of [3H]cytochalasin B with high affinity. The results of this study indicate that both villin and cytochalasin B bind to the same end of actin filaments, yet differ in their binding sites.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochalasin B,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/villin
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
257
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
395-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7053377-Actins,
pubmed-meshheading:7053377-Animals,
pubmed-meshheading:7053377-Calcium,
pubmed-meshheading:7053377-Carrier Proteins,
pubmed-meshheading:7053377-Cytochalasin B,
pubmed-meshheading:7053377-Erythrocyte Membrane,
pubmed-meshheading:7053377-Humans,
pubmed-meshheading:7053377-Kinetics,
pubmed-meshheading:7053377-Macromolecular Substances,
pubmed-meshheading:7053377-Microfilament Proteins,
pubmed-meshheading:7053377-Muscles,
pubmed-meshheading:7053377-Rabbits
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pubmed:year |
1982
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pubmed:articleTitle |
Interaction of cytochalasin B with actin filaments nucleated or fragmented by villin.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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