Linked Life Data

7049249

Source:http://linkedlifedata.com/resource/pubmed/id/7049249

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-10-12
pubmed:abstractText
Escherichia coli asparaginase (L-asparagine amidohydrolase, EC 3.5.1.1) has been modified by succinylation, acetylation and the attachment of N,N-dimethyl-1,3-propanediamine and glucuronic acid. The effect of these modifications on plasma clearance rates in mice and on other properties is compared to the effects of modification with lactose and N-acetylneuraminyl lactose studied previously. The t 1/2 values for the acylated enzyme samples (lower pI) were reduced, succinylated asparaginase sharply and the acetylated enzyme less so. The N,N-dimethyl-1,3-propanediamine-modified samples (increased pI) also had lower t 1/2 values, but samples modified with glucuronic acid (reduced pI) showed little change in clearance time. The main conclusion is that the increased t 1/2 value found in the previous work for N-acetylneuraminyl-lactosylated enzyme is not due to the decreased pI value of the modified enzyme, but must be attributed to interference by N-acetylneuraminyl-lactose residues, directly or indirectly, with the mechanism normally used by the mouse to clear itself of injected E. coli asparaginase.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
704
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
345-52
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Chemical modifications of Escherichia coli L-asparaginase and their effect on plasma clearance rate and other properties.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.