Linked Life Data

7049243

Source:http://linkedlifedata.com/resource/pubmed/id/7049243

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-10-29
pubmed:abstractText
The peptide composition of plasma membrane in Saccharomyces cerevisiae cells growing at different temperatures between 18 and 38 degrees C was studied using SDS-polyacrylamide gel electrophoresis. Stability of the proteins, both qualitative and quantitative, was observed at the tested temperatures. Treatment for 2 h with cycloheximide decreased by about 50% the amount of a 80 kDa membrane peptide at 18, 23, 28 and 33 degrees C, with no other apparent effects. At 38 degrees C the 80 kDa peptide was not affected by the presence of the drug. Addition of tunicamycin to cultures at concentrations partially inhibitory to growth caused a large accumulation of the 80 kDa peptide in the plasma membrane, which cycloheximide did not modify. Pulse-chase experiments indicated a low rate of turnover of total plasma membranes in cells growing at 18 and 28 degrees C. In contrast, at 38 degrees C about 50% of the radioactivity in plasma membranes disappeared after a 2 h chase. The 80 kDa protein band was the only one with significant differential decay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
689
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
38-44
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Turnover of protein components of the plasma membrane of Saccharomyces cerevisiae.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't