Linked Life Data

7047183

Source:http://linkedlifedata.com/resource/pubmed/id/7047183

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1982-9-10
pubmed:abstractText
Marrow-derived adherent cells (MDAC) have been shown to provide a microenvironment which supports the proliferation of hematopoietic stem cells in continuous bone marrow culture. A study was undertaken to investigate the origin of MDAC. Normal CBA mice were transplanted with marrow cells from CBA donors bearing two T6 chromosomes. Five weeks after transplantation, the mice were sacrificed, their marrow cells explanted in liquid culture and the numbers of T6 positive and T6 negative mitoses were monitored during cultivation of MDAC. After 6 weeks, established MDAC monolayers were recharged with second explants of marrow cells and their capacity to support HSC proliferation in continuous marrow culture was assessed. The number of T6 positive mitoses declined steadily during the period of MDAC cultivation and none were detected after 4 weeks. The MDAC monolayers were able to support in vitro proliferation of CFUs for periods of at least 5 weeks. These results suggest that the in vitro hematopoietic microenvironment was of recipient origin and therefore stromal.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0301-472X
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
367-72
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
The origin of the hematopoietic microenvironment in continuous bone marrow culture.
pubmed:publicationType
Journal Article