Linked Life Data

7045272

Source:http://linkedlifedata.com/resource/pubmed/id/7045272

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-8-14
pubmed:abstractText
Monoclonal antibodies were raised against sporozoites of two species of malaria parasites, Plasmodium falciparum and Plasmodium vivax. The antibodies reacted with polypeptides (circumsporozoite proteins) that are uniformly distributed over the entire surface of sporozoites, as shown by indirect immunofluorescence and by the circumsporozoite precipitin reaction. The epitopes recognized by the monoclonal antibodies were expressed on sporozoites from different geographical isolates of the homologous species but were not detected on sporozoites of heterologous species nor on blood forms of the parasite. The monoclonal antibody to P. falciparum specifically immunoprecipitated two polypeptides of apparent 67,000 mol wt (Pf67) and 58,000 mol wt (Pf58) from extracts of [35S]methionine-labeled P. falciparum sporozoites. Similarly, the anti-P. vivax monoclonal immunoprecipitated two proteins of 51,000 mol wt (Pv51) and 45,000 mol wt (Pv45) from extracts of metabolically labeled P. vivax sporozoites. The extracts were also reacted with the serum of human volunteers successfully vaccinated with sporozoites of either P. vivax or P. falciparum. The patterns of immunoprecipitation were almost identical to those obtained with the corresponding monoclonal antibodies. The circumsporozoite proteins of P. falciparum and P. vivax play a role in immune protection. Incubation of the appropriate monoclonal antibody with viable sporozoites of the homologous species significantly reduced parasite infectivity, as determined by sporozoite neutralization assays carried out in splenectomized chimpanzees.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-1102604, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-1172191, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-120770, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-14284906, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-320027, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-4583408, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-4590095, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-4987036, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-4987037, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-6080011, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-6785357, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-6991628, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-7026723, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-7037957, http://linkedlifedata.com/resource/pubmed/commentcorrection/7045272-825374
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
156
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
20-30
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Circumsporozoite proteins of human malaria parasites Plasmodium falciparum and Plasmodium vivax.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't