Linked Life Data

7045100

Source:http://linkedlifedata.com/resource/pubmed/id/7045100

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1982-8-7
pubmed:abstractText
RNA synthesized by reovirus cores was not capped or methylated when GTP was replaced by guanosine 5'-O-(3-thiotriphosphate) [gamma-S]GTP as a reaction substrate. Full length transcripts were synthesized in the presence of [gamma-S]GTP which indicated that capping could be dissociated from the process of transcription. Exogenous [gamma-S]GTP was not cleaved by the virion-associated phosphohydrolase and the majority of transcripts contained triphosphorylated 5'-termini. [gamma-S]GTP did, however, act as a donor for the capping of RNA chains initiated with GTP. Chromatography on mercury-agarose of products initiated with [gamma-S]GTP resulted in the binding of only the small (14 S) size class of RNA, although transcripts not bound to the affinity column were also thiophosphorylated.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
257
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7018-22
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Guanosine 5'-O-(3-thiotriphosphate) inhibits capping of reovirus mRNA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't