Linked Life Data

7043998

Source:http://linkedlifedata.com/resource/pubmed/id/7043998

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10-11
pubmed:dateCreated
1982-7-8
pubmed:abstractText
A technique for the preparation of relatively pure myoblasts from chick primary culture is described. Cultured rat myogenic cells (L6) were plated and grown to provide three morphologically distinct cell populations: perfusion, postfusion, and nonfusion. Homogenates of L6 cells demonstrated two major peaks of proteolytic activity at pH 3.0 and 5.5. The activity could be partially inhibited by leupeptin or pepstatin. Differential centrifugation indicated significant acid hydrolase activity in the "H" fraction of prefused cells, which shifted to the "L" fraction after fusion of the cells. Two populations of lysosomes were resolved in the myoblasts and myotubes after isopycnic centrifugation in Percoll. The equilibrium densities were 1.044 and 1.060-1.068. Cells were incubated with several protease inhibitors. Only chloroquine caused a large inhibition of protein degradation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0001-5318
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1333-47
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Properties of the lysosomal apparatus during differentiation of cultured striated muscle cells.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't