Linked Life Data

7038682

Source:http://linkedlifedata.com/resource/pubmed/id/7038682

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1982-5-21
pubmed:abstractText
Purkinje neurons of the cerebellar cortex from a chemically and morphologically heterogeneous population containing some members that have gamma-aminobutyric acid (GABA), others that have immunoreactivity for motilin, and a small number that have both. The remaining 30-40% of all Purkinje cells have neither of these two neuroactive substances, leaving possibilities for other transmitter candidates. The evidence was compiled from double-staining immunocytochemical procedures performed on single sections of the cerebellum and brain stem in rat, mouse, and monkey. Two polyclonal antibodies were applied in succession, one directed against the midregion and COOH terminus of the 22-amino acid polypeptide motilin and the other against glutamic acid decarboxylase (glutamate decarboxylase; L-glutamate 1-carboxy-lyase, EC 4.1.1.15), the rate-limiting enzyme in the synthesis of the neurotransmitter GABA. The staining combinations employed the immunoperoxidase method, with different chromogens for distinguishing the motilin-like immunoreactivity from glutamic acid decarboxylase immunoreactivity by different colors, or the immunoperoxidase method for one antiserum and immunofluorescence for the other. The locations of both motilin and GABA cell types were mapped. The recognition of motilin in Purkinje cells calls for experimental definition of the role of this substance in the cerebellum and for reevaluation of the roles of Purkinje cells and of GABA in cerebellar function. The significant motilin representation in the flocculus, paraflocculus, and vermis suggests that it may be the Purkinje cell mediative chemical in the vestibular parts of the cerebellum. However, the presence of GABA as well in the same regions indicates that the chemical preference may be at least bimodal.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-1089460, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-116858, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-13574437, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-208082, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-273213, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-287044, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-374176, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-4179831, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-474996, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-4773166, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-4854839, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-4941085, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-5717715, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-5718512, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-598441, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-6020387, http://linkedlifedata.com/resource/pubmed/commentcorrection/7038682-92901
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7787-91
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Chemical heterogeneity in cerebellar Purkinje cells: existence and coexistence of glutamic acid decarboxylase-like and motilin-like immunoreactivities.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't