Linked Life Data

7034599

Source:http://linkedlifedata.com/resource/pubmed/id/7034599

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1982-3-26
pubmed:abstractText
Two strains of Escherichia coli were opsonized by incubation in heat-inactivated bovine blood serum and in whey. The opsonized bacteria were then immobilized by complexing with anti-bovine antibodies previously coated to walls of polystyrene tubes. The amount of bovine immunoglobulin (Ig) G in the immobilized complex was then determined by a direct enzyme-linked immunosorbent assay, using peroxidase as enzyme. Thus, a direct measurement of one class of opsonic substances on the surface of the organisms was determined. The sensitivity for measurement of IgG ranged from nanograms to micrograms. After incubation in blood serum, 500-fold more IgG was found on the surface of the serum-resistant strain. The amount of IgG absorbed from whey in each instance was much less than that absorbed from serum. The number of bound molecules per bacterium ranged between 2,000 and 2,000,000, depending on both the strain and the serum.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0002-9645
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1735-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Enzyme-linked immunosorbent assay for the quantitation of immunoglobulin G bound to Escherichia coli.
pubmed:publicationType
Journal Article, Comparative Study