| pubmed-article:7033395 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7033395 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:7033395 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:7033395 | lifeskim:mentions | umls-concept:C0019764 | lld:lifeskim |
| pubmed-article:7033395 | lifeskim:mentions | umls-concept:C1179149 | lld:lifeskim |
| pubmed-article:7033395 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:7033395 | pubmed:dateCreated | 1982-3-22 | lld:pubmed |
| pubmed-article:7033395 | pubmed:abstractText | All nucleated cells express HLA-A, B, and C antigens. However, only a few cells, including epidermal cells, demonstrate HLA-DR antigens which are potent transplantation immunogens in man. The current study was undertaken to determine if epidermal cell continue to synthesize and/or express HLA-DR antigens after prolonged in vitro culture. Epidermal cells cultured for 7 days or more no longer stimulated allogeneic lymphocytes in the epidermal cell-lymphocyte reaction. Indirect immunofluorescence light microscopy of cultured cells using mouse monoclonal antibody to HLA-DR antigen confirmed that these cells do not express HLA-DR antigens whereas they retain beta 2-microglobulin. Detergent extracts of 12-day cultured epidermal cells biosynthetically labeled with 35 S-methionine were immunoprecipitated with monoclonal anti-DR antibody and analyzed by the method of two-dimensional polyacrylamide gel electrophoresis. No radiolabeled proteins were found on these gels in the regions where HLA-DR molecules are known to migrate. These data indicate that HLA-DR antigen is absent from cultured epidermal cells. Finally, we describe a technique for growing epidermal cells on a gelatin membrane which allows subsequent removal of intact cell monolayers from the culture dish. Such monolayers may be useful for purposes of transplantation. | lld:pubmed |
| pubmed-article:7033395 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7033395 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7033395 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7033395 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7033395 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7033395 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7033395 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7033395 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7033395 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7033395 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:7033395 | pubmed:issn | 0022-202X | lld:pubmed |
| pubmed-article:7033395 | pubmed:author | pubmed-author:EnglemanE GEG | lld:pubmed |
| pubmed-article:7033395 | pubmed:author | pubmed-author:BenikeC JCJ | lld:pubmed |
| pubmed-article:7033395 | pubmed:author | pubmed-author:CoxA JAJ | lld:pubmed |
| pubmed-article:7033395 | pubmed:author | pubmed-author:CharronD JDJ | lld:pubmed |
| pubmed-article:7033395 | pubmed:author | pubmed-author:MorhennV BVB | lld:pubmed |
| pubmed-article:7033395 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7033395 | pubmed:volume | 78 | lld:pubmed |
| pubmed-article:7033395 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7033395 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7033395 | pubmed:pagination | 32-7 | lld:pubmed |
| pubmed-article:7033395 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7033395 | pubmed:meshHeading | pubmed-meshheading:7033395-... | lld:pubmed |
| pubmed-article:7033395 | pubmed:meshHeading | pubmed-meshheading:7033395-... | lld:pubmed |
| pubmed-article:7033395 | pubmed:meshHeading | pubmed-meshheading:7033395-... | lld:pubmed |
| pubmed-article:7033395 | pubmed:meshHeading | pubmed-meshheading:7033395-... | lld:pubmed |
| pubmed-article:7033395 | pubmed:meshHeading | pubmed-meshheading:7033395-... | lld:pubmed |
| pubmed-article:7033395 | pubmed:meshHeading | pubmed-meshheading:7033395-... | lld:pubmed |
| pubmed-article:7033395 | pubmed:meshHeading | pubmed-meshheading:7033395-... | lld:pubmed |
| pubmed-article:7033395 | pubmed:meshHeading | pubmed-meshheading:7033395-... | lld:pubmed |
| pubmed-article:7033395 | pubmed:meshHeading | pubmed-meshheading:7033395-... | lld:pubmed |
| pubmed-article:7033395 | pubmed:meshHeading | pubmed-meshheading:7033395-... | lld:pubmed |
| pubmed-article:7033395 | pubmed:meshHeading | pubmed-meshheading:7033395-... | lld:pubmed |
| pubmed-article:7033395 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:7033395 | pubmed:articleTitle | Cultured human epidermal cells do not synthesize HLA-DR. | lld:pubmed |
| pubmed-article:7033395 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7033395 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7033395 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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