Linked Life Data

7031644

Source:http://linkedlifedata.com/resource/pubmed/id/7031644

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1982-2-12
pubmed:abstractText
Experiments were carried out on a freshwater sponge (Ephydatia mulleri) in order to demonstrate the presence of fibronectin in Porifera. By using antibodies to highly purified human plasma fibronectin, the presence of a similar or identical protein could be demonstrated in the membranes of E. mulleri cells such as epithelial cells, fibroblast-like cells, and choanocytes. The reaction was specific, could be abolished by the addition of excess fibronectin, and was not observed with nonimmune rabbit serum. The immune fluorescent reaction became stronger when the sponge cells were pretreated with acetone and could also be observed, although with a less intense staining, on the intercellular matrix. This shows the predominant presence of a sponge fibronectin-like protein in the cell membranes and also its presence to a lesser extent in the intercellular matrix. When dissociated sponge cells were led to reassociate under the microscope, reassociation could be completely inhibited by anti-human fibronectin antiserum up to a dilution of 1:120 and partially inhibited up to a dilution of 1:240. The reassociation of dissociated sponge cells could also be inhibited by the addition of purified gelatin but not with serum albumin or with a normal, nonimmune rabbit serum. These results clearly indicate that a sponge cell fibronectin-like protein may play an important role as the (or one of the) recognition site(s) of the aggregation factor(s) and can therefore be directly involved in cell association, morphogenesis, and differentiation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7031644-1097576, http://linkedlifedata.com/resource/pubmed/commentcorrection/7031644-357987, http://linkedlifedata.com/resource/pubmed/commentcorrection/7031644-361081, http://linkedlifedata.com/resource/pubmed/commentcorrection/7031644-4203032, http://linkedlifedata.com/resource/pubmed/commentcorrection/7031644-4473270, http://linkedlifedata.com/resource/pubmed/commentcorrection/7031644-5157350, http://linkedlifedata.com/resource/pubmed/commentcorrection/7031644-534500, http://linkedlifedata.com/resource/pubmed/commentcorrection/7031644-5650605, http://linkedlifedata.com/resource/pubmed/commentcorrection/7031644-6456720, http://linkedlifedata.com/resource/pubmed/commentcorrection/7031644-803256, http://linkedlifedata.com/resource/pubmed/commentcorrection/7031644-925079
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6261-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Fibronectin-like protein in Porifera: its role in cell aggregation.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.