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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1982-1-28
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pubmed:abstractText |
The leucine-specific binding protein of Escherichia coli is a periplasmic protein that is synthesized as a precursor and subsequently is processed during its secretion into the periplasmic space. The processing of both the leucine-specific binding protein and a plasmid-coded beta-lactamase is inhibited by phenethyl alcohol and by the proton ionophore, carbonylcyanide m-chlorophenylhydrazone (CCCP). The levels of CCCP that inhibit processing also produce significant decreases in the membrane potential. Valinomycin, a potassium ionophore, also inhibits processing of the leucine-specific binding protein in spheroplasts. Processing can be restored in CCCP-treated cells and in valinomycin-treated spheroplasts by dilution of the treated cells in fresh medium. These results suggest a role for membrane potential in the secretion of periplasmic proteins. A model is presented which suggests that membrane potential plays a primary role in the proper orientation of the precursor signal sequence within the membrane, thus promoting processing and secretion.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-159284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-172125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-354502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-405218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-4527943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-4555955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-4570991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-4604283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-6253830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-6264431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-6448092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-6928682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-6929499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-6990419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-6993100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-6995457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-7001463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-7007385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-7012452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7029533-7018904
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carbonyl Cyanide m-Chlorophenyl...,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/LivK protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/Periplasmic Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Valinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Lactamases
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5396-400
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7029533-Bacterial Proteins,
pubmed-meshheading:7029533-Carbonyl Cyanide m-Chlorophenyl Hydrazone,
pubmed-meshheading:7029533-Carrier Proteins,
pubmed-meshheading:7029533-Escherichia coli,
pubmed-meshheading:7029533-Escherichia coli Proteins,
pubmed-meshheading:7029533-Membrane Potentials,
pubmed-meshheading:7029533-Models, Biological,
pubmed-meshheading:7029533-Periplasmic Binding Proteins,
pubmed-meshheading:7029533-Potassium Chloride,
pubmed-meshheading:7029533-Protein Precursors,
pubmed-meshheading:7029533-Valinomycin,
pubmed-meshheading:7029533-beta-Lactamases
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pubmed:year |
1981
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pubmed:articleTitle |
Role for membrane potential in the secretion of protein into the periplasm of Escherichia coli.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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