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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1977-10-28
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pubmed:abstractText |
A cytomegalovirus (CMV) induced lymphocyte tranformation test (LTT) performed as a microtechnique assay is described. Investigations were done in a group of twenty-five healthy adults with normal lymphocyte reactivity to phytohaemagglutinin and previously encountered antigens. Lymphocyte reactivity was established in CMV-seropositive persons using heat-inactivated cell-free CMV strain AD 169 in an optimal stimulating dose of 10(1-5) TCID 50. Positive CMV-LTT results were shown to be induced by purified inactivated CMV. Most persons (twelve out of seventeen) with antibodies against CMV-induced late or virus-structure antigens (LA) showed positive (stimulation index greater than 2-0) CMV-LTT results. Magnitude of CMV-LTT responses was positively related to the CMV-LA Ab titres (P less than 0-01). All persons (six) with antibodies against CMV early or non-structural antigens showed positive CMV-LTT results and highest stimulation rates were found in this group. Generally CMV-seronegative persons were non-responsive. Only in one case (LA Ab titre 10) a borderline positive response (stimulation index 2-3) was seen. The CMV-LTT was shown to have virus-specific properties because it was not related to simultaneously performed LTTs with Epstein-Barr virus and Herpes simplex virus in the same persons. It is concluded that CMV-LTTs are useful virus-specific in vitro techniques for the study of CMV-directed lymphocyte reactivity. Its possible relationship to other cell-mediated immunity tests and its practical applications are discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-1080258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-163788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-164405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-177371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-180190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-186434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-4128996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-4143245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-4160230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-4278135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-4325893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-4335071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-4340084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-4342032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-4343614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-4355279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-4360547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-4605055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-4913804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/70294-765017
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
52-60
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:70294-Adult,
pubmed-meshheading:70294-Antibodies, Viral,
pubmed-meshheading:70294-Antigens, Viral,
pubmed-meshheading:70294-Cytomegalovirus,
pubmed-meshheading:70294-Dose-Response Relationship, Immunologic,
pubmed-meshheading:70294-Epitopes,
pubmed-meshheading:70294-Female,
pubmed-meshheading:70294-Humans,
pubmed-meshheading:70294-Lymphocyte Activation,
pubmed-meshheading:70294-Lymphocytes,
pubmed-meshheading:70294-Male,
pubmed-meshheading:70294-Middle Aged
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pubmed:year |
1977
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pubmed:articleTitle |
Cytomegalovirus-directed lymphocyte reactivity in healthy adults tested by a CMV-induced lymphocyte transformation test.
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pubmed:publicationType |
Journal Article
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