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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1981-11-18
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pubmed:abstractText |
A solid-phase radioimmunoassay was developed to measure serum IgG antibodies to a purified protein antigen I/II prepared from Streptococcus mutans. The assay was specific to this antigen and significant binding of 125I-radiolabelled antiserum was found only in sera from rhesus monkeys immunized with the antigen I/II but not in sham-immunized monkeys or those immunized with streptococcal antigen III. A very significant correlation was found in serum IgG antibodies tested by the radioimmunoassay and an immunofluorescent technique (r = 0.88, P less than 0.001). The sensitivity of the double-layer radioimmunoassay was increased 10 times by the addition of a third antibody layer and this enabled gingival crevicular fluid antibodies to be measured. Comparison of paired samples of serum and crevicular fluid revealed a very significant correlation between IgG antibodies to streptococcal antigen I/II in the the two fluids (P less than 0.001). These findings suggest that serum antibodies can reach the tooth surface via gingival crevicular fluid.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-104924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-1063145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-1080067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-116635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-130476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-14450081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-287422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-393961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-4146880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-4501483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-4735375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-6060361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-6103387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-6154101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-6995311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-7419244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-76131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-804000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-819360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-826825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7023762-93576
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
417-24
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7023762-Animals,
pubmed-meshheading:7023762-Antibodies, Bacterial,
pubmed-meshheading:7023762-Antibody Specificity,
pubmed-meshheading:7023762-Bacterial Proteins,
pubmed-meshheading:7023762-Fluorescent Antibody Technique,
pubmed-meshheading:7023762-Gingival Crevicular Fluid,
pubmed-meshheading:7023762-Humans,
pubmed-meshheading:7023762-Immunoglobulin G,
pubmed-meshheading:7023762-Macaca mulatta,
pubmed-meshheading:7023762-Radioimmunoassay,
pubmed-meshheading:7023762-Streptococcus mutans
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pubmed:year |
1981
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pubmed:articleTitle |
A radioimmunoassay for serum and gingival crevicular fluid antibodies to a purified protein of Streptococcus mutans.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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