Linked Life Data

7019717

Source:http://linkedlifedata.com/resource/pubmed/id/7019717

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5820
pubmed:dateCreated
1981-9-15
pubmed:abstractText
The ampC gene of Escherichia coli K-12 codes for a beta-lactamase which can hydrolyse the beta-lactam ring of ampicillin. Ampicillin resistance is strictly related to ampC gene copy number thus we have been able to isolate ampicillin-resistant mutants carrying multiple ampC repeats. We have isolated on a plasmid a segment of chromosomal DNA carrying multiple ampC repeats, and compared the nucleotide sequence of the region joining repeat units to the sequence of the DNA segments that fused to create the joint. The fusion had occurred within a 12-base pair (bp) sequence of perfect homology. We suggest that recombination between randomly occurring short homologies (12-13-bp long), could be a general mechanism to generate tandem duplications in the size range of 10 kilobases (kb).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
292
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
269-71
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Recombination between short DNA homologies causes tandem duplication.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't