Linked Life Data

7017709

Source:http://linkedlifedata.com/resource/pubmed/id/7017709

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-8-20
pubmed:abstractText
We describe a method by which complex protein mixtures are fractionated by standard one-dimensional Na-DodSO4/polyacrylamide gel electrophoresis or O'Farrell two-dimensional gel electrophoresis and then are efficiently and rapidly transferred electrophoretically to diazobenzyloxymethyl- or diazophenylthioether-paper and analyzed by immunoautoradiography. The method is illustrated with protein extracts of human KB cells infected with adenovirus type 2. Proteins were transferred from gels without decrease in resolution and with an increase in the sensitivity of detection by autoradiography when [35S]-methionine-labeled proteins were used. When unlabeled proteins were transferred, low levels of virus encoded proteins could be detected by sequential treatment of diazobenzyloxymethyl-paper with anti-adenovirus type 2 virion or anti-73,000 DNA binding protein and 125I-labeled Staphyloccus aureus protein A. Covalently bound viral proteins retained immunologic reactivity after dissociation of the protein A and antibody, By one-dimensional gel transfer/immunoautoradiography, seven virion proteins were detected as prominent bands and several others as weaker bands. By two-dimensional gel transfer/immunoautoradiography, several additional viral proteins were detected. By use of anti-DNA binding protein serum, the Mr 73,000 protein and Mr 41,000-48,000 subspecies were detected. A protein present at a concentration of approximately 1 part in 100,000 of the total protein can be identified in cell extracts. This method may be applicable to various biological problems requiring resolution and detection of small amounts of specific proteins that can be recognized immunologically or that can be detected by binding to specific radiolabeled DNA or RNA sequences or hormones.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017709-1102604, http://linkedlifedata.com/resource/pubmed/commentcorrection/7017709-1258364, http://linkedlifedata.com/resource/pubmed/commentcorrection/7017709-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/7017709-215191, http://linkedlifedata.com/resource/pubmed/commentcorrection/7017709-236308, http://linkedlifedata.com/resource/pubmed/commentcorrection/7017709-351332, http://linkedlifedata.com/resource/pubmed/commentcorrection/7017709-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/7017709-414220, http://linkedlifedata.com/resource/pubmed/commentcorrection/7017709-423779, http://linkedlifedata.com/resource/pubmed/commentcorrection/7017709-473597, http://linkedlifedata.com/resource/pubmed/commentcorrection/7017709-6243775, http://linkedlifedata.com/resource/pubmed/commentcorrection/7017709-64628, http://linkedlifedata.com/resource/pubmed/commentcorrection/7017709-7425302, http://linkedlifedata.com/resource/pubmed/commentcorrection/7017709-91164
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
177-81
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Immunoautoradiographic detection of proteins after electrophoretic transfer from gels to diazo-paper: analysis of adenovirus encoded proteins.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.