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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1981-8-27
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pubmed:abstractText |
The subset of B lymphocytes having IgG on their surfaces was purified from human spleen and blood using a fluorescence-activated cell sorter (FACS). This subset constituted about 15% of B lymphocytes. The remaining non-IgG-bearing B cells were also obtained for study. These two populations were examined for (a) their expression of other surface immunoglobulin isotypes, (b) their ability to give rise to IgG- and IgM-secreting (plaque-forming) cells in a pokeweed mitogen (PWM)-driven culture system, and (c) their ability to proliferate in response to PWM stimulation. The results of these studies indicate that most IgG-bearing B cells also express surface IgM and IgD. Less than 15% had only IgG. The IgG-positive cell gave rise to both IgG and IgM plaque-forming cells when driven by PWM, and in fact were responsible for most of the total plaque response in both the IgG and IgM classes. The non-IgG-bearing B cells were depleted of both IgG and IgM responsiveness. The failure of the non-IgG-bearing B cells to give a strong response to PWM did not appear to be due to either depletion of accessory cells or to any suppressive influence. Finally, proliferation studies indicated that both the IgG-bearing and the non-IgG-bearing cells proliferated in the presence of PWM with a somewhat stronger proliferative response in the IgG-bearing cells. These results demonstrate that the IgG-bearing cell is not irreversibly committed to IgG production but can also give rise to IgM-secreting cells, and that human PWM-driven immunoglobulin secretory responses are predominantly due to a numerically small subset of B cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-1083417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-1083418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-1086788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-112737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-301171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-301535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-306414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-311144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-313424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-323402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-323403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-326451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-359206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-385776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-4536706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-4542737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-489970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-67148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-6966197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-789096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-803528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-804005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7017061-89732
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
153
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
339-51
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7017061-B-Lymphocytes,
pubmed-meshheading:7017061-Cell Communication,
pubmed-meshheading:7017061-Cell Division,
pubmed-meshheading:7017061-Hemolytic Plaque Technique,
pubmed-meshheading:7017061-Humans,
pubmed-meshheading:7017061-Immunoglobulin G,
pubmed-meshheading:7017061-Immunoglobulin M,
pubmed-meshheading:7017061-Pokeweed Mitogens,
pubmed-meshheading:7017061-Receptors, Antigen, B-Cell,
pubmed-meshheading:7017061-Spleen
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pubmed:year |
1981
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pubmed:articleTitle |
Human B lymphocyte subsets. I. IgG-bearing B cell response to pokeweed mitogen.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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