Linked Life Data

7014722

Source:http://linkedlifedata.com/resource/pubmed/id/7014722

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1981-7-23
pubmed:abstractText
A new method is described for detection of membrane antigens and antibodies against such structures. By inserting partially purified rat transplantation antigen (RT-1) into iodine or fluorescein-labeled lipid vesicles, a precipitation of the liposome is demonstrated by the use of a specific alloantiserum and a heterologous anti-rat IgG serum. Precipitation of liposomes carrying WF transplantation antigens could be detected at a final dilution of the alloantiserum of 1:3000, which is comparable to that obtained in a 51Cr-release assay. Furthermore, when unpurified membrane proteins from WF splenocytes were incorporated into labeled liposomes, an amount of RT-1 corresponding to 8000 cells could be detected.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
351-63
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Liposome immune assay (LIA). Use of membrane antigens inserted into labeled lipid vesicles as targets in immune assays.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't