Linked Life Data

7013412

Source:http://linkedlifedata.com/resource/pubmed/id/7013412

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1981-6-25
pubmed:abstractText
Immunofluorescence (IF) techniques performed on formol-fixed, paraffin-embedded and protease-pretreated sections from autopsy brain for demonstration of rabies and Herpes simplex type 1 (HSV-1) antigens proved to be a simple and effective method to ensure the etiology of some viral encephalitides. In contrast to fresh material, the use of formol-fixed tissues rules out any risk in handling highly infectious material. Another major advantage is the possibility of comparing IF appearance with normal histology of the same section by post-staining with hematoxilineosin (H.E.) or other stains. In 2 animal and one of 2 human rabies cases, neuronal cytoplasmic inclusions were more prominent in IF than in H.E. stains. In 31 cases with a histopathological diagnosis of necrotizing encephalitis, HSV-1 antigen was demonstrated in 17 of 25 acute cases but absent in 6 cases with clinical courses longer than 4 weeks. Specific fluorescence of nuclear inclusions bodies was inconstant and less prominent than that of neuronal cytoplasm and neuronal/glial nuclear membranes. Since HSV-1 positive cells are patchily distributed, limited tissue sampling (e.g. in diagnostic biopsies) may yield false negative results. Paucity of HSV-1-positive cells in the leptomeninges renders CSF immunocytology of limited use for diagnostic purposes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0065-1435
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
142-4
pubmed:dateRevised
2007-11-9
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Immunohistological studies in viral encephalitis.
pubmed:publicationType
Journal Article