Linked Life Data

7012086

Source:http://linkedlifedata.com/resource/pubmed/id/7012086

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1981-6-13
pubmed:abstractText
The uptake of polystyrene latex beads (approximately 0.75 micrometers) and glutaraldehyde-treated erythrocytes by human corneal stromal keratocytes maintained in culture has been studied. Combined phase-contrast and scanning electron microscopic observations on individual cells after exposure to either beads or erythrocytes demonstrated that the majority of these particles were present intracellularly. Transmission electron microscopy revealed that the beads were membrane-bound within the cytoplasm of these cells. Thus the human keratocytes in culture are phagocytic and able to internalize particles ranging in size from approximately 0.75 to 6 micrometer. Kinetic studies showed continuous uptake of the polystyrene latex beads for at least 72 hr, with an approximate linear uptake rate between 4 and 48 hr. The extent of bead uptake was dependent on the initial bead concentration. The human keratocyte cultures were markedly more phagocytic than human skin fibroblasts or rabbit chondrocytes. It was also found that after extensive bead uptake the normal growth pattern of the keratocytes was affected. It is suggested that the phagocytic ability of the human keratocytes is involved in the turnover of the corneal stromal matrix as well as in the initial response of this avascular tissue to injury or bacterial infection.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0146-0404
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
481-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Phagocytic properties of human keratocyte cultures.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.