Linked Life Data

7011394

Source:http://linkedlifedata.com/resource/pubmed/id/7011394

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-6-13
pubmed:abstractText
A general method for the labelling and recovery of platelet membrane proteins is described. This method is based on the principle of labelling cell membrane proteins with reagents containing haptenic groups. The labelled proteins can then be isolated by affinity chromatography, using antibodies against the haptenic groups on the labelling reagents. Two compounds representing different groups of reagents were synthesized and used: 2,4-dinitrophenyl-beta-alanine hydrazide, which is a labelling reagent for glycoproteins, and diazodiiodoarsanilic acid, which binds to externally exposed membrane proteins. 2,4-Dinitrophenyl-beta-alanine hydrazide binds to aldehydes formed from oxidized sialic acid. Three main proteins were isolated using this reagent. Using diazodiiodoarsanilic acid, a protein having a molecular weight of 85,000 was labelled and later isolated in an affinity column with anti-arsanilic acid antibodies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
641
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
114-21
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Isolation of platelet plasma membrane proteins by affinity chromatography.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't