Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1981-5-21
|
| pubmed:abstractText |
From citrate-utilizing (Cit+) Escherichia coli strain C53 of bovine origin, strains C53A and C53B were obtained. Upon mating with recA+ but not with recA mutant recipients of K-12, C53A produced chromosomal recombinants at quite high frequencies, leading to the following conclusions: (i) C53A is an Hfr strain; (ii) the site of integration of the Cit plasmid (IncH1) is between metA (89 min) and ara (1 min); (iii) the direction of chromosome transfer is clockwise; and (iv) the plasmid-associated determinants are transferred as the terminal markers. A transductant of a dnaA(Ts) strain, CRT46, which acquired Cit determinants from a recombinant, SG13, was also an Hfr strain similar to SG13, and thermoresistant due to suppressive integration. On the other hand, unstable C53B did not produce recombinants, but the frequency of RecA-independent transfer of the Cit plasmid was high, indicating that the Cit plasmid (IncH1) exists autonomously in C53B. Attempts to isolate an Hfr strain from C53B failed.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7009550-13267987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7009550-340919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7009550-349354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7009550-357936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7009550-370344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7009550-396889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7009550-4597997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7009550-4599123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7009550-6100891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7009550-7012413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7009550-773363
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0021-9193
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
143
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1090-4
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading | |
| pubmed:year |
1980
|
| pubmed:articleTitle |
Spontaneous emergence of an Hfr strain with a cit plasmid from natural isolates of citrate-positive Escherichia coli bovine origin.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|