Linked Life Data

7005212

Source:http://linkedlifedata.com/resource/pubmed/id/7005212

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-3-24
pubmed:abstractText
A mutant in the Escherichia coli lac permease, called Yf, appears to be defective in the biogenesis and proper assembly of this membrane protein. It was proposed that this defect led to the accumulation of a precursor of the mutant permease (Fried, V. A. (1977) J. Mol Biol. 114, 477-490). In this communication, evidence is presented that the lacYf mutant accumulates a novel lac-specific soluble polypeptide with a molecular weight of 87,000. Detected by double-label analysis on sodium dodecyl sulfate gels, and identified as a lac-specific polypeptide on a two-dimensional gel system, this polypeptide is immunoprecipitated by anti-transacetylase antibody. Pulse-chase experiments are consistent with the hypothesis that it is converted in vivo into a lac-specific membrane protein with an apparent molecular weight of 28,000, which appears to be the mutant lac permease. The results suggest that the 87,000-dalton soluble protein is a precursor of the mutant lac permease. It is proposed that this precursor is a polyprotein chimera containing both the lacY and lacA gene products.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
256
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
244-52
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Membrane biogenesis. Evidence that a soluble chimeric polypeptide can serve as a precursor of a mutant lac permease in Escherichia coli.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.