Linked Life Data

7003792

Source:http://linkedlifedata.com/resource/pubmed/id/7003792

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-2-19
pubmed:abstractText
Heterozygotes for the mutant genes crooked (Cd) or rib fusion (Rf), crossed to either strain A/J or SWV produce mutant F1 offspring with minor skeletal defects. If the mothers are also treated with teratogenic doses of insulin, the mutant F1 heterozygotes develop exencephaly significantly more often than nonmutants. That the heterozygous mutant gene increased the embryo's sensitivity to insulin-induced exencephaly was also demonstrated by dose-response curves. For A/J and SWV crosses, probit regression lines for the mutant crosses were fitted to common slopes that were significantly different from those of probit regression lines for the nonmutant crosses. The nonparallelism of the mutant versus the nonmutant groups of lines indicated the presence of a gene-teratogen interaction. Early exencephalic embryos showed delayed turning in one-third of treated mutant crosses and less than a quarter of treated nonmutant crosses. Another one-third of exencephalics, in treated mutant crosses only, had abnormally twisted hindbodies and appeared to be phenocopies of untreated homozygotes. Insulin treatment significantly slowed the rates of embryo turning and neural tube closure but did not affect somite number. Histologically, insulin did not appear to cause cell death, and mitotic index of mesoderm was unaffected. Only exencephalic embryos showed a significant reduction in neurectoderm mitotic index. Insulin caused a reduction in neurectoderm cell proliferation, delay in pattern of turning and delay in neural tube closure to the greatest extent in exencephalic embryos. It was postulated that extensive delays in turning could provide a mechanical basis for the failure of the neural tube to close.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0040-3709
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
125-39
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Gene-teratogen interaction in insulin-induced mouse exencephaly.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't